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pspa family 1 clade 1  (ATCC)


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    ATCC pspa family 1 clade 1
    Pspa Family 1 Clade 1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pspa+clades/Streptococcus+pneumoniae+(Klein)+Chester/pm29959043-114-24-21
    Average 95 stars, based on 145 article reviews
    pspa family 1 clade 1 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Correlation between In Vitro Complement Deposition and Passive Mouse Protection of Anti-Pneumococcal Surface Protein A Monoclonal Antibodies
    Article Snippet: .. In this study, we analyzed 18 IgG anti-PspA hkR36A MAbs for the extent of cross-reactivity with recombinant PspA belonging to the 6 PspA clades (i.e., PspA L82016 , PspA R36A , PspA TIGR4 , PspA JCP#56 , PspA ATCC 6303 , and PspA BG9300 ) by an ELISA ( ). ..

    Article Title: Correlation between In Vitro Complement Deposition and Passive Mouse Protection of Anti-Pneumococcal Surface Protein A Monoclonal Antibodies
    Article Snippet: .. In this study, we analyzed 18 IgG anti-PspA hkR36A MAbs for the extent of cross-reactivity with recombinant PspA belonging to the 6 PspA clades (i.e., PspA L82016 , PspA R36A , PspA TIGR4 , PspA JCP#56 , PspA ATCC 6303 , and PspA BG9300 ) by an ELISA ( ). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Correlation between In Vitro Complement Deposition and Passive Mouse Protection of Anti-Pneumococcal Surface Protein A Monoclonal Antibodies
    Article Snippet: .. In this study, we analyzed 18 IgG anti-PspA hkR36A MAbs for the extent of cross-reactivity with recombinant PspA belonging to the 6 PspA clades (i.e., PspA L82016 , PspA R36A , PspA TIGR4 , PspA JCP#56 , PspA ATCC 6303 , and PspA BG9300 ) by an ELISA ( ). ..

    Article Title: Correlation between In Vitro Complement Deposition and Passive Mouse Protection of Anti-Pneumococcal Surface Protein A Monoclonal Antibodies
    Article Snippet: .. In this study, we analyzed 18 IgG anti-PspA hkR36A MAbs for the extent of cross-reactivity with recombinant PspA belonging to the 6 PspA clades (i.e., PspA L82016 , PspA R36A , PspA TIGR4 , PspA JCP#56 , PspA ATCC 6303 , and PspA BG9300 ) by an ELISA ( ). ..



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    Image Search Results


    Schematic diagram of PspA/Rx1 fragments 1 to 7 (F1 to F7, respectively) (adapted from reference ). At the top is shown the whole mature PspA molecule, containing the N-terminal αHD (including regions A and B), the proline-rich domain (C), and the choline-binding domain at the C terminus. In the center of the proline-rich domain is a nonproline block (N) surrounded by proline-rich sequences (P). The PspA leader (not shown) is removed during PspA expression by pneumococci. The B region of the αHD has been called the clade-determining region . Also shown are six overlapping 81- or 100-amino-acid fragments covering the α-helical and proline-rich domains. The sequence positions of the first and final amino acids of each fragment are listed at the right.

    Journal: mSphere

    Article Title: The Modified Surface Killing Assay Distinguishes between Protective and Nonprotective Antibodies to PspA

    doi: 10.1128/mSphere.00589-19

    Figure Lengend Snippet: Schematic diagram of PspA/Rx1 fragments 1 to 7 (F1 to F7, respectively) (adapted from reference ). At the top is shown the whole mature PspA molecule, containing the N-terminal αHD (including regions A and B), the proline-rich domain (C), and the choline-binding domain at the C terminus. In the center of the proline-rich domain is a nonproline block (N) surrounded by proline-rich sequences (P). The PspA leader (not shown) is removed during PspA expression by pneumococci. The B region of the αHD has been called the clade-determining region . Also shown are six overlapping 81- or 100-amino-acid fragments covering the α-helical and proline-rich domains. The sequence positions of the first and final amino acids of each fragment are listed at the right.

    Article Snippet: Peptide arrays of the αHD of PspA/Rx1 (PspA clade 2), composed of 15-amino-acid peptides containing 11-amino-acid overlaps, were obtained from CelluSpots, Intavis, Germany, and used as previously described ( ).

    Techniques: Binding Assay, Blocking Assay, Expressing, Sequencing

    Reactivity of MAbs with native PspA, 81- or 100-aa peptides of PspA, and the PspA PRD

    Journal: mSphere

    Article Title: The Modified Surface Killing Assay Distinguishes between Protective and Nonprotective Antibodies to PspA

    doi: 10.1128/mSphere.00589-19

    Figure Lengend Snippet: Reactivity of MAbs with native PspA, 81- or 100-aa peptides of PspA, and the PspA PRD

    Article Snippet: Peptide arrays of the αHD of PspA/Rx1 (PspA clade 2), composed of 15-amino-acid peptides containing 11-amino-acid overlaps, were obtained from CelluSpots, Intavis, Germany, and used as previously described ( ).

    Techniques: Enzyme-linked Immunosorbent Assay

    ELISA-based analysis for reactivity of anti-PspA hkR36A MAbs with recombinant PspAs representing the 6 PspA clades a

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Correlation between In Vitro Complement Deposition and Passive Mouse Protection of Anti-Pneumococcal Surface Protein A Monoclonal Antibodies

    doi: 10.1128/CVI.00001-14

    Figure Lengend Snippet: ELISA-based analysis for reactivity of anti-PspA hkR36A MAbs with recombinant PspAs representing the 6 PspA clades a

    Article Snippet: In this study, we analyzed 18 IgG anti-PspA hkR36A MAbs for the extent of cross-reactivity with recombinant PspA belonging to the 6 PspA clades (i.e., PspA L82016 , PspA R36A , PspA TIGR4 , PspA JCP#56 , PspA ATCC 6303 , and PspA BG9300 ) by an ELISA ( ).

    Techniques: Enzyme-linked Immunosorbent Assay, Recombinant

    Surface binding of anti-PspAR36A polyclonal sera with pneumococcal strains representing the 6 clades of PspA. Surface binding with strains BG8838 (A), D39 (B), TIGR4 (C), EF5668 (D), ATCC 6303 (E), and BG6803 (F) was analyzed by flow cytometry using anti-PspAR36A polyclonal and preimmune sera. Preimmune serum (solid gray histogram) was used as the negative control. The GMFI values for the anti-PspAR36A polyclonal and preimmune sera are shown. GMFI values of ≥2 times the value obtained with the preimmune sera were considered significant.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Correlation between In Vitro Complement Deposition and Passive Mouse Protection of Anti-Pneumococcal Surface Protein A Monoclonal Antibodies

    doi: 10.1128/CVI.00001-14

    Figure Lengend Snippet: Surface binding of anti-PspAR36A polyclonal sera with pneumococcal strains representing the 6 clades of PspA. Surface binding with strains BG8838 (A), D39 (B), TIGR4 (C), EF5668 (D), ATCC 6303 (E), and BG6803 (F) was analyzed by flow cytometry using anti-PspAR36A polyclonal and preimmune sera. Preimmune serum (solid gray histogram) was used as the negative control. The GMFI values for the anti-PspAR36A polyclonal and preimmune sera are shown. GMFI values of ≥2 times the value obtained with the preimmune sera were considered significant.

    Article Snippet: In this study, we analyzed 18 IgG anti-PspA hkR36A MAbs for the extent of cross-reactivity with recombinant PspA belonging to the 6 PspA clades (i.e., PspA L82016 , PspA R36A , PspA TIGR4 , PspA JCP#56 , PspA ATCC 6303 , and PspA BG9300 ) by an ELISA ( ).

    Techniques: Binding Assay, Flow Cytometry, Negative Control